Deep and fast live imaging with two-photon scanned light-sheet microscopy

Nat Methods. 2011 Jul 17;8(9):757-60. doi: 10.1038/nmeth.1652.

Abstract

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila melanogaster
  • Embryo, Nonmammalian / ultrastructure*
  • Imaging, Three-Dimensional / instrumentation
  • Imaging, Three-Dimensional / methods*
  • Lasers
  • Light / adverse effects
  • Microscopy / methods*
  • Zebrafish