Reduction of HSV-1 binding to BHK cells after treatment with phosphatidylinositol-specific phospholipase C

N Langeland; L J Moore

doi:10.1016/0014-5793(90)80859-h

Reduction of HSV-1 binding to BHK cells after treatment with phosphatidylinositol-specific phospholipase C

FEBS Lett. 1990 Dec 17;277(1-2):253-6. doi: 10.1016/0014-5793(90)80859-h.

Authors

N Langeland¹, L J Moore

Affiliation

¹ Department of Biochemistry, Haukeland Hospital, University of Bergen, Norway.

PMID: 2176619
DOI: 10.1016/0014-5793(90)80859-h

Abstract

Baby-hamster kidney cells were treated with unspecific and phosphatidylinositol-specific phospholipase C (PI-PLC) prior to infection with herpes simplex virus type 1 or 2. Subsequent to PI-PLC treatment, a 30% reduction of infectivity and receptor binding was observed for type 1 virus, while type 2 was unaffected. Treating the cells with unspecific phospholipase C did not affect subsequent infection with either virus. Treatment of the virus particles with the unspecific phospholipase reduced its infectivity, probably due to loss of the viral envelope. PI-PLC treatment of virus particles did not have any such effect on virus infectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cricetinae
In Vitro Techniques
Membrane Proteins / metabolism
Phosphatidylinositols / metabolism
Receptors, Virus / metabolism*
Simplexvirus / metabolism*
Type C Phospholipases / pharmacology*

Substances

Membrane Proteins
Phosphatidylinositols
Receptors, Virus
Type C Phospholipases