Structural mass spectrometry of proteins using hydroxyl radical based protein footprinting

Anal Chem. 2011 Oct 1;83(19):7234-41. doi: 10.1021/ac200567u. Epub 2011 Aug 1.


Structural MS is a rapidly growing field with many applications in basic research and pharmaceutical drug development. In this feature article the overall technology is described and several examples of how hydroxyl radical based footprinting MS can be used to map interfaces, evaluate protein structure, and identify ligand dependent conformational changes in proteins are described.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Hydroxyl Radical / chemistry*
  • Mass Spectrometry
  • Models, Molecular
  • Protein Conformation
  • Protein Footprinting*
  • Proteins / chemistry*


  • Proteins
  • Hydroxyl Radical