Establishment and characterization of a primitive neuroectodermal tumor of bone continuous cell line (LAP-35)

Int J Cell Cloning. 1990 Nov;8(6):409-24. doi: 10.1002/stem.5530080644.


A continuous tumor cell line (LAP-35) was established from a primitive neuroectodermal tumor of bone from the right tibia of a 12-year-old female. The neural character of the cell line was documented by the spontaneous growth of neurites and by the presence of several neural markers, including neuron-specific enolase (NSE), S-100 protein, neurofilaments, chromogranin A, synaptophysin and positivity to monoclonal antibodies UJ127.11, UJ13A, UJ181.4. Cell-sorter analysis showed a high expression of nerve growth factor receptor (NGFr) and major histocompatibility complex class I-related molecules. A unique cytogenetic profile was observed, including a reciprocal chromosomal translocation (rct) 11:22 (q24;q12), typically associated with Ewing's sarcoma and neuroepithelioma, and deletion of the short arm of chromosome 1 (lp-), otherwise a feature of neuroblastoma. N-myc proto-oncogene was neither amplified nor expressed, whereas the expression of c-myc was documented by northern blot analysis. These features distinguish this new cell line from previously reported neuroectodermal cell lines, identifying LAP-35 as a unique model of a group of neural bone tumors that share characteristics of neuroblastoma as well as neuroepithelioma.

MeSH terms

  • Animals
  • Bone Neoplasms / pathology*
  • Cell Division / physiology
  • Child
  • Female
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Genes, myc / genetics
  • Humans
  • Immunohistochemistry
  • Karyotyping
  • Mice
  • Mice, Nude
  • Microscopy, Electron
  • Neoplasm Transplantation
  • Neoplasms, Nerve Tissue / pathology*
  • Tumor Cells, Cultured / cytology*