In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts

Asian Pac J Trop Med. 2011 Mar;4(3):201-4. doi: 10.1016/S1995-7645(11)60069-2. Epub 2011 Apr 12.


Objective: To evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (Moringa oleifera) on the growth of gram-positive and negative bacteria.

Methods: Paper disks were soaked with 100, 200, 300 and 400 μL of extract at 20 g/180 mL and 10 g/190 mL. All extracts were tested against Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923), Vibrio parahaemolyticus, Enterococcus faecalis (ATCC29212), Pseudomonas aeruginosa (ATCC27853), Salmonella enteritidis (IH) and Aeromonas caviae. The susceptibility tests were performed using the modified disk diffusion method.

Results: The strains E. coli, P. aeruginosa and S. enteritidis (IH) were resistant to all treatments. In general, disks with 400 μL extract were the most efficient against S. aureus, V. parahaemolyticus, E. faecalis and A. caviae.

Conclusions: The study indicates a promising potential for aqueous and ethanolic Moringa leaf extracts as alternative treatment of infections caused by the tested strains.

MeSH terms

  • Anti-Bacterial Agents / isolation & purification*
  • Anti-Bacterial Agents / pharmacology*
  • Bacteria / drug effects*
  • Bacteria / growth & development
  • Humans
  • Microbial Sensitivity Tests / methods
  • Moringa oleifera / chemistry*
  • Plant Extracts / isolation & purification*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry


  • Anti-Bacterial Agents
  • Plant Extracts