Purification of tubulin from porcine brain

Methods Mol Biol. 2011;777:15-28. doi: 10.1007/978-1-61779-252-6_2.

Abstract

Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Swine
  • Tubulin / isolation & purification*

Substances

  • Tubulin