Variations on the single-molecule assay for microtubule-associated proteins and kinesins

Methods Mol Biol. 2011;777:167-76. doi: 10.1007/978-1-61779-252-6_12.

Abstract

The direct observation of single kinesins and microtubule-associated proteins (MAPs) has become a core tool for cytoskeleton research. We outline several variations to the core experiment that allow the researcher to explore structural and biophysical mechanisms underlying kinesin motility and MAP function.

MeSH terms

  • Kinesins / chemistry*
  • Kinesins / metabolism
  • Microscopy, Fluorescence
  • Microtubule-Associated Proteins / chemistry*
  • Microtubule-Associated Proteins / metabolism

Substances

  • Microtubule-Associated Proteins
  • Kinesins