DNA/NYVAC vaccine regimen induces HIV-specific CD4 and CD8 T-cell responses in intestinal mucosa

J Virol. 2011 Oct;85(19):9854-62. doi: 10.1128/JVI.00788-11. Epub 2011 Jul 20.


In the present study, we have investigated the anatomic distribution in blood and gut mucosal tissues of memory poxvirus-specific CD4 and CD8 T cells in subjects vaccinated with smallpox and compared it with vector (NYVAC)-specific and HIV insert-specific T-cell responses induced by an experimental DNA-C/ NYVAC-C vaccine regimen. Smallpox-specific CD4 T-cell responses were present in the blood of 52% of the subjects studied, while smallpox-specific CD8 T cells were rarely detected (12%). With one exception, smallpox-specific T cells were not measurable in gut tissues. Interestingly, NYVAC vector-specific and HIV-specific CD4 and CD8 T-cell responses were detected in almost 100% of the subjects immunized with DNA-C/NYVAC-C in blood and gut tissues. The large majority (83%) of NYVAC-specific CD4 T cells expressed α4β7 integrins and the HIV coreceptor CCR5. These results demonstrate that the experimental DNA-C/NYVAC-C HIV vaccine regimen induces the homing of potentially protective HIV-specific CD4 and CD8 T cells in the gut, the port of entry of HIV and one of the major sites for HIV spreading and the depletion of CD4 T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / administration & dosage
  • AIDS Vaccines / immunology*
  • Adult
  • Blood / immunology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Humans
  • Intestinal Mucosa / immunology*
  • Middle Aged
  • Smallpox Vaccine / administration & dosage
  • Smallpox Vaccine / immunology*
  • Vaccination / methods
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / immunology*
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*


  • AIDS Vaccines
  • NYVAC vaccine
  • Smallpox Vaccine
  • Vaccines, DNA
  • Viral Vaccines