Calcitonin gene-related peptide (CGRP) receptors are linked to cyclic adenosine monophosphate production in SK-N-MC human neuroblastoma cells

Neurosci Lett. 1990 Nov 13;119(2):195-8. doi: 10.1016/0304-3940(90)90832-t.


Calcitonin gene-related peptide (CGRP) stimulated cyclic adenosine monophosphate (cAMP) levels in SK-N-MC human neuroblastoma cells in a time- and concentration-dependent manner. The efficacy order for CGRPs was human alpha-CGRP = human beta-CGRP = chick CGRP greater than rat CGRP greater than human [Tyr0]CGRP. Calcitonin (CT) failed to influence cAMP production in SK-N-MC cells. [Tyr0]CGRP27-37 which by itself did not affect cAMP levels antagonized CGRP action. Saturation analysis using [125I]CGRP showed a homogeneous population of binding sites. CGRP but not CT, vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) inhibited radioligand binding. Our results provide evidence that human neuroblastoma SK-N-MC cells contain highly specific CGRP receptors which are positively coupled to cAMP generation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Binding, Competitive
  • Calcitonin Gene-Related Peptide / metabolism*
  • Calcitonin Gene-Related Peptide / pharmacology
  • Cyclic AMP / biosynthesis*
  • Humans
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • Neuropeptide Y / pharmacology
  • Receptors, Cell Surface / metabolism*
  • Tumor Cells, Cultured / metabolism*
  • Tumor Cells, Cultured / pathology
  • Vasoactive Intestinal Peptide / pharmacology


  • Neuropeptide Y
  • Receptors, Cell Surface
  • Vasoactive Intestinal Peptide
  • Cyclic AMP
  • Calcitonin Gene-Related Peptide