From unwinding to clamping - the DEAD box RNA helicase family

Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16. doi: 10.1038/nrm3154.


RNA helicases of the DEAD box family are present in all eukaryotic cells and in many bacteria and Archaea. These highly conserved enzymes are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DEAD box proteins use ATP to unwind short duplex RNA in an unusual fashion and remodel RNA-protein complexes, but they can also function as ATP-dependent RNA clamps to provide nucleation centres that establish larger RNA-protein complexes. Structural, mechanistic and molecular biological studies have started to reveal how these conserved proteins can perform such diverse functions and how accessory proteins have a central role in their regulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Binding Sites
  • DEAD-box RNA Helicases / chemistry
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Eukaryotic Initiation Factor-4A / metabolism
  • Humans
  • Models, Biological
  • Models, Molecular
  • Nucleocytoplasmic Transport Proteins / metabolism
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / metabolism


  • Nucleocytoplasmic Transport Proteins
  • RNA, Messenger
  • Adenosine Triphosphate
  • Eukaryotic Initiation Factor-4A
  • DEAD-box RNA Helicases