Thyrotropin activates both the cyclic AMP and the PIP2 cascades in CHO cells expressing the human cDNA of TSH receptor

Mol Cell Endocrinol. 1990 Nov 12;74(1):R1-6. doi: 10.1016/0303-7207(90)90209-q.


The effect of thyrotropin (TSH) on cyclic AMP accumulation, phosphatidylinositol bisphosphate (PIP2) hydrolysis and [Ca2+]i rise has been studied in CHO cells stably transfected with human TSH receptor (hTSHR) cDNA. In human thyroid slices, TSH activates these two intracellular cascades with a higher affinity for the adenylate cyclase activation (from 0.1 to 1 mU/ml TSH) than for phospholipase C activation (from 1 to 10 mU/ml TSH). The CHO cells transfected with the recently cloned cDNA of human TSH receptor respond in the same way to TSH. They respond between 0.1 and 1 mU/ml TSH for cyclic AMP accumulation and between 1 and 10 mU/ml TSH for inositol monophosphate (IP1) increase. In these same cells, TSH 10 mU/ml, but not forskolin (10 microM), or dibutyryl cyclic AMP (100 microM), clearly enhances intracellular calcium concentration [( Ca2+]i). Our results demonstrate unequivocally that a single transcription unit has the potential to encode receptor molecules coupled to both cascades.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Animals
  • Bucladesine / pharmacology
  • Calcium / metabolism
  • Cell Line
  • Colforsin / pharmacology
  • Cricetinae
  • Cyclic AMP / metabolism*
  • DNA / metabolism
  • Enzyme Activation
  • Gene Expression Regulation*
  • Humans
  • Inositol Phosphates / metabolism
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositols / metabolism*
  • Receptors, Thyrotropin / genetics*
  • Thyrotropin / pharmacology*
  • Transfection
  • Type C Phospholipases / metabolism


  • Inositol Phosphates
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositols
  • Receptors, Thyrotropin
  • Colforsin
  • Bucladesine
  • Thyrotropin
  • DNA
  • Cyclic AMP
  • Type C Phospholipases
  • Adenylyl Cyclases
  • Calcium