Nucleolar proteins with altered expression in leukemic cell lines

Leuk Res. 2012 Feb;36(2):232-6. doi: 10.1016/j.leukres.2011.06.038. Epub 2011 Jul 23.


The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Blotting, Western
  • Cell Nucleolus / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Fluorescent Antibody Technique
  • Humans
  • Nuclear Proteins / metabolism*
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / diagnosis*
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism*
  • Prohibitins
  • Repressor Proteins / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Two-Dimensional Difference Gel Electrophoresis


  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • Nuclear Proteins
  • PHB protein, human
  • Prohibitins
  • Repressor Proteins