Turnover of ATP synthase subunits in F1-depleted HeLa and yeast cells

FEBS Lett. 2011 Aug 19;585(16):2582-6. doi: 10.1016/j.febslet.2011.07.011. Epub 2011 Jul 23.


Mitochondrial translation of the Saccharomyces cerevisiae Atp6p subunit of F(1)-F(0) ATP synthase is regulated by the F(1) ATPase. Here we show normal expression of Atp6p in HeLa cells depleted of the F(1) β subunit. Instead of being translationally down-regulated, HeLa cells lacking F(1) degrade Atp6p, thereby preventing proton leakage across the inner membrane. Mammalian mitochondria also differ in the way they minimize the harmful effect of unassembled F(1) α subunit. While yeast mutants lacking β subunit have stable aggregated F(1) α subunit in the mitochondrial matrix, the human α subunit is completely degraded in cells deficient in F(1) β subunit. These results are discussed in light of the different properties of the proteins and environments in which yeast and human mitochondria exist.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cattle
  • HeLa Cells
  • Humans
  • Mitochondria / enzymology
  • Mitochondria / metabolism
  • Mitochondrial Proton-Translocating ATPases / biosynthesis
  • Mitochondrial Proton-Translocating ATPases / metabolism*
  • Protein Biosynthesis
  • Protein Subunits / chemistry
  • Protein Subunits / deficiency*
  • Protein Subunits / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / enzymology*
  • Solubility


  • MT-ATP6 protein, human
  • Protein Subunits
  • Mitochondrial Proton-Translocating ATPases