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. 2011 Oct;1813(10):1827-35.
doi: 10.1016/j.bbamcr.2011.06.022. Epub 2011 Jul 8.

Induction of the Intrinsic Apoptosis Pathway in Insulin-Secreting Cells Is Dependent on Oxidative Damage of Mitochondria but Independent of caspase-12 Activation

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Induction of the Intrinsic Apoptosis Pathway in Insulin-Secreting Cells Is Dependent on Oxidative Damage of Mitochondria but Independent of caspase-12 Activation

Ilir Mehmeti et al. Biochim Biophys Acta. .
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Abstract

Pro-inflammatory cytokine-mediated beta cell apoptosis is activated through multiple signaling pathways involving mitochondria and endoplasmic reticulum. Activation of organelle-specific caspases has been implicated in the progression and execution of cell death. This study was therefore performed to elucidate the effects of pro-inflammatory cytokines on a possible cross-talk between the compartment-specific caspases 9 and 12 and their differential contribution to beta cell apoptosis. Moreover, the occurrence of ROS-mediated mitochondrial damage in response to beta cell toxic cytokines has been quantified. ER-specific caspase-12 was strongly activated in response to pro-inflammatory cytokines; however, its inhibition did not abolish cytokine-induced mitochondrial caspase-9 activation and loss of cell viability. In addition, there was a significant induction of oxidative mitochondrial DNA damage and elevated cardiolipin peroxidation in insulin-producing RINm5F cells and rat islet cells. Overexpression of the H(2)O(2) detoxifying enzyme catalase effectively reduced the observed cytokine-induced oxidative damage of mitochondrial structures. Taken together, the results strongly indicate that mitochondrial caspase-9 is not a downstream substrate of ER-specific caspase-12 and that pro-inflammatory cytokines cause apoptotic beta cell death through activation of caspase-9 primarily by hydroxyl radical-mediated mitochondrial damage.

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