Antioxidant acitivity of low molecular weight hyaluronic acid

Food Chem Toxicol. 2011 Oct;49(10):2670-5. doi: 10.1016/j.fct.2011.07.020. Epub 2011 Jul 20.


Two polysaccharides, low molecular weight hyaluronic acid-1 (LMWHA-1) and LMWHA-2, with their molecular weight of 1.45×10(5) and 4.52×10(4)Da, respectively, were prepared from high molecular weight hyaluronic acid (HA,1.05×10(6)Da). LMWHA-1, LMWHA-2 and HA were studied for their antioxidant activities. In vitro antioxidant assay, LMWHA showed strong inhibition of lipid peroxidation and scavenging activities of hydroxyl radical, moderate 1,1-diphenyl-2-picryldydrazyl radical and superoxide anion scavenging activity. In addition, the LMWHA-1 exhibited much stronger antioxidant activity than LMWHA-2 and HA. For antioxidant testing in vivo, LMWHA-1, LMWHA-2 and HA were orally administrated over a period of 7days in a cyclophosphamide(CY) induced immunosuppressed mice model. As results, administration of LMWHA was able to overcome CY-induced immunosuppression and significantly raised the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) in immunosuppressed mice. The results showed that the LMWHA, possessing pronounced free radical scavenging and antioxidant activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Biphenyl Compounds / metabolism
  • Catalase / blood
  • Free Radical Scavengers / pharmacology*
  • Glutathione Peroxidase / blood
  • Hyaluronic Acid / pharmacology*
  • Immunocompromised Host
  • Male
  • Malondialdehyde / blood
  • Mice
  • Oxidative Stress / drug effects
  • Picrates / metabolism
  • Random Allocation
  • Superoxide Dismutase / blood


  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Picrates
  • Malondialdehyde
  • Hyaluronic Acid
  • 1,1-diphenyl-2-picrylhydrazyl
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase