Exercise modulates the insulin-induced translocation of glucose transporters in rat skeletal muscle

FEBS Lett. 1990 Feb 26;261(2):256-60. doi: 10.1016/0014-5793(90)80566-2.

Abstract

Insulin and acute exercise (45 min of treadmill run) increased glucose uptake into perfused rat hindlimbs 5-fold and 3.2-fold, respectively. Following exercise, insulin treatment resulted in a further increase in glucose uptake. The subcellular distribution of the muscle glucose transporters GLUT-1 and GLUT-4 was determined in plasma membranes and intracellular membranes. Neither exercise nor exercise----insulin treatment altered the distribution of GLUT-1 transporters in these membrane fractions. In contrast, exercise, insulin and exercise----insulin treatment caused comparable increases in GLUT-4 transporters in the plasma membrane. The results suggest that exercise might limit insulin-induced GLUT-4 recruitment and that following exercise, insulin may alter the intrinsic activity of plasma membrane glucose transporters.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Membrane / metabolism
  • Cytochalasin B / metabolism
  • Glucose / metabolism
  • Insulin / pharmacology*
  • Male
  • Monosaccharide Transport Proteins / metabolism*
  • Muscles / drug effects
  • Muscles / metabolism*
  • Physical Exertion / physiology*
  • Rats
  • Rats, Inbred Strains

Substances

  • Insulin
  • Monosaccharide Transport Proteins
  • Cytochalasin B
  • Glucose