The purpose of the study was to determine the vitamin content of twenty standardized dishes commonly consumed in Nigeria. Representative samples of twenty Nigerian dishes were analyzed for fat-soluble (vitamins A, D, E, and K) and water-soluble (vitamin C and B- complexes) vitamins. The foods analyzed included those based on cereals, starchy tubers and roots, legumes, and vegetables. The analysis was carried out using spectrophotometry. The results revealed the following concentrations (mg/100 g): vitamin C , undetectable to 2.692 mg/100 g; thiamine, 0.011-1.094 mg/100 g; riboflavin, 0.011-0.816 mg/100 g; pyridoxine, undetectable to 0.412 mg/100 g; niacin, 0.070-0.967 mg/100 g; pantothenic acid, 0.060-1.193 mg/100 g; biotin, undetectable to 2.092 mg/100 g; B12, 0.045-2.424 µg/100 g; folate, 7.822-101.764 µg/100 g; total vitamin A, undetectable to 121.444 µg RE/100 g; vitamin D, undetectable to 2.445 IU/100 g; vitamin E, undetectable to 2.627 IU/100 g; and vitamin K, 0.266-13.091 µg/100 g. The results suggest that these dishes are good sources of fat-soluble and water-soluble vitamins and will provide baseline data that will be valuable in complementing available food composition data, and in estimating dietary intake of vitamins in Nigeria.