Sec14l3 is specifically expressed in mouse airway ciliated cells

Inflammation. 2012 Apr;35(2):702-12. doi: 10.1007/s10753-011-9363-z.


Airway epithelium is a key component for airway integrity. Previously, we found that expression of the Sec14l3 gene that encodes a 45-kDa secretory protein is inversely associated with the progression of experimentally induced airway inflammation and degeneration/necrosis of alveolar epithelium. In this report, using in situ hybridization we demonstrated that the ciliated cells in mouse lung selectively express Sec14l3 mRNA. In a three-dimensional culture of mouse tracheal epithelial cells, levels of the Sec14l3 mRNA correlated with the differentiation of ciliated cells. Intranasal infection of adult mice with influenza virus resulted in a 20-fold, progressive decrease in Sec14l3 mRNA expression over 10 days post infection. These results enhance the potential value of Sec14l3 as a ciliated epithelial cell-specific biomarker for the progression of airway inflammations such as airway viral infection and asthma.

MeSH terms

  • Animals
  • Biomarkers
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Differentiation
  • Cells, Cultured
  • Epithelial Cells / metabolism*
  • Epithelial Cells / virology
  • Female
  • Influenza A Virus, H1N1 Subtype
  • Lung / cytology
  • Lung / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / metabolism
  • Pulmonary Alveoli / cytology
  • Pulmonary Alveoli / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Respiratory Mucosa / cytology
  • Respiratory Mucosa / metabolism*
  • Trachea / cytology
  • Trachea / metabolism*


  • Biomarkers
  • Carrier Proteins
  • RNA, Messenger
  • Sec14l3 protein, mouse