Identification of a Tc1-like transposon integration site in the genome of the flounder (Platichthys flesus): a novel use of an inverse PCR method

Mar Genomics. 2010 Mar;3(1):45-50. doi: 10.1016/j.margen.2010.03.001. Epub 2010 May 5.

Abstract

The inverse PCR method has been developed and applied employed for the identification of the integration sites of the Tc1-like transposons in the genome of the flounder, Platichthys flesus. One Tc1-like insertion instance was recognized and characterized, demonstrating an efficiency of the method for determining of transposon integration sites. The similarity of the sequence flanking transposon (SFT) to reverse transcriptase sequences (RVT) was demonstrated. It is likely that the insertion took place within currently degenerated LINE (long interspersed nuclear elements) retrotransposon.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cluster Analysis
  • Computational Biology
  • DNA Primers / genetics
  • DNA Transposable Elements / genetics*
  • DNA-Binding Proteins / genetics
  • Flounder / genetics*
  • Models, Genetic
  • Molecular Sequence Data
  • Phylogeny*
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Species Specificity
  • Terminal Repeat Sequences / genetics
  • Transposases / genetics

Substances

  • DNA Primers
  • DNA Transposable Elements
  • DNA-Binding Proteins
  • Tc1 transposase
  • Transposases