The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10

J Biosci. 2011 Aug;36(3):425-38. doi: 10.1007/s12038-011-9089-y.

Abstract

The conserved band-interband pattern is thought to reflect the looped-domain organization of insect polytene chromosomes. Previously, we have shown that the chromodomain protein Chriz and the zinc-finger protein Z4 are essentially required for the maintenance of polytene chromosome structure. Here we show that both proteins form a complex that recruits the JIL-1 kinase to polytene chromosomes, enabling local H3S10 phosphorylation of interband nucleosomal histones. Interband targeting domains were identified at the N-terminal regions of Chriz and Z4, and our data suggest partial cooperation of the complex with the BEAF boundary element protein in polytene and diploid cells. Reducing the core component Chriz by RNAi results in destabilization of the complex and a strong reduction of interband-specific histone H3S10 phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Proteins / metabolism*
  • DNA-Binding Proteins / metabolism
  • Drosophila / genetics
  • Drosophila / metabolism*
  • Drosophila Proteins / metabolism*
  • Eye Proteins / metabolism
  • Histones / metabolism*
  • Interphase
  • Nuclear Matrix-Associated Proteins / metabolism*
  • Phosphorylation
  • Polytene Chromosomes / metabolism*
  • Protein-Serine-Threonine Kinases / metabolism*

Substances

  • BEAF-32 protein, Drosophila
  • Cell Cycle Proteins
  • Chro protein, Drosophila
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Eye Proteins
  • Histones
  • Nuclear Matrix-Associated Proteins
  • pzg protein, Drosophila
  • JIL-1 protein, Drosophila
  • Protein-Serine-Threonine Kinases