Oil body proteins sequentially accumulate throughout seed development in Brassica napus

J Plant Physiol. 2011 Nov 15;168(17):2015-20. doi: 10.1016/j.jplph.2011.06.007. Epub 2011 Jul 31.


Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination [DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (~25 DAP) onwards.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica napus / growth & development
  • Brassica napus / metabolism*
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism
  • Fatty Acids / metabolism
  • Gene Expression
  • Phospholipids / metabolism
  • Plant Oils / metabolism*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Proteomics
  • Seeds / growth & development
  • Seeds / metabolism*
  • Time Factors
  • Triglycerides / metabolism


  • Calcium-Binding Proteins
  • Fatty Acids
  • Phospholipids
  • Plant Oils
  • Plant Proteins
  • Triglycerides
  • caleosin protein, Sesamum indicum
  • oleosin protein, Brassica napus