Amplified fragment length polymorphism analysis of Listeria monocytogenes by Experion™ automated microfluidic electrophoresis

J Microbiol Methods. 2011 Oct;87(1):119-24. doi: 10.1016/j.mimet.2011.07.011. Epub 2011 Jul 23.

Abstract

Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis / instrumentation
  • Amplified Fragment Length Polymorphism Analysis / methods*
  • Automation, Laboratory
  • Bacterial Proteins / genetics
  • Electrophoresis, Microchip / instrumentation
  • Electrophoresis, Microchip / methods*
  • Food Microbiology
  • Humans
  • Listeria monocytogenes / classification
  • Listeria monocytogenes / genetics*
  • Listeriosis / microbiology
  • Molecular Typing / instrumentation
  • Molecular Typing / methods*
  • Phylogeny

Substances

  • Bacterial Proteins
  • internalin protein, Bacteria