Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC

Talanta. 2011 Sep 15;85(3):1466-71. doi: 10.1016/j.talanta.2011.06.027. Epub 2011 Jun 29.

Abstract

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Chromatography, High Pressure Liquid / instrumentation
  • Chromatography, High Pressure Liquid / methods*
  • Creatinine / blood*
  • Humans
  • Hydrogen-Ion Concentration
  • Kynurenine / blood*
  • Neopterin / blood*
  • Phosphates / chemistry
  • Reproducibility of Results
  • Tryptophan / blood*

Substances

  • Buffers
  • Phosphates
  • Kynurenine
  • Neopterin
  • Tryptophan
  • Creatinine