Highly selectively monitoring heavy and transition metal ions by a fluorescent sensor based on dipeptide

Talanta. 2011 Sep 15;85(3):1566-74. doi: 10.1016/j.talanta.2011.06.052. Epub 2011 Jun 24.

Abstract

Fluorescent sensor (DMH) based on dipeptide was efficiently synthesized in solid phase synthesis. The dipeptide sensor shows sensitive response to Ag(I), Hg(II), and Cu(II) among 14 metal ions in 100% aqueous solution. The fluorescent sensor differentiates three heavy metal ions by response type; turn on response to Ag(I), ratiometric response to Hg(II), and turn off detection of Cu(II). The detection limits of the sensor for Ag(I) and Cu(II) were much lower than the EPA's drinking water maximum contaminant levels (MCL). Specially, DMH penetrated live cells and detected intracellular Ag(+) by turn on response. We described the fluorescent change, binding affinity, detection limit for the metal ions. The study of a heavy metal-responsive sensor based on dipeptide demonstrates its potential utility in the environment field.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • Copper / analysis
  • Copper / chemistry
  • Dipeptides / chemical synthesis
  • Dipeptides / chemistry*
  • Fluorescence
  • HeLa Cells
  • Humans
  • Intracellular Space / chemistry
  • Ions / chemistry
  • Mercury / analysis
  • Mercury / chemistry
  • Metals, Heavy / chemistry*
  • Reproducibility of Results
  • Silver / analysis
  • Silver / chemistry
  • Solid-Phase Synthesis Techniques
  • Spectrometry, Fluorescence / methods*
  • Transition Elements / chemistry*
  • Water Pollutants, Chemical / analysis

Substances

  • Dipeptides
  • Ions
  • Metals, Heavy
  • Transition Elements
  • Water Pollutants, Chemical
  • Silver
  • Copper
  • Mercury