The effects of bFGF, IGF-I, and TGF-beta on RMo skeletal muscle cell proliferation and differentiation

Exp Cell Res. 1990 Apr;187(2):250-4. doi: 10.1016/0014-4827(90)90088-r.

Abstract

A new skeletal muscle cell line, rat myoblast omega or RMo, has been characterized with regard to the effects of three growth factors: basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), and transforming growth factor beta (TGF-beta). Results indicate a differential response of these factors on both cell proliferation and differentiation. Exposure to bFGF and IGF-I stimulate proliferation, while TGF-beta has no effect on cell number. RMo cell differentiation, as indicated by skeletal myosin synthesis, is enhanced by IGF-I, whereas both bFGF and TGF-beta suppress differentiation. These responses are in agreement with the effects of bFGF, IGF-I, and TGF-beta on myogenic cells cultured from fetal and postnatal muscle, thereby suggesting that RMo cells can serve as a model system for the study of growth factor effects on skeletal muscle cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • DNA Replication / drug effects
  • Fibroblast Growth Factors / pharmacology*
  • Immunoenzyme Techniques
  • Insulin-Like Growth Factor I / pharmacology*
  • Kinetics
  • Muscles / cytology*
  • Muscles / drug effects
  • Rats
  • Somatomedins / pharmacology*
  • Thymidine / metabolism
  • Transforming Growth Factors / pharmacology*
  • Tritium

Substances

  • Somatomedins
  • Tritium
  • Fibroblast Growth Factors
  • Insulin-Like Growth Factor I
  • Transforming Growth Factors
  • Thymidine