Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae

Exp Cell Res. 1990 Apr;187(2):315-9. doi: 10.1016/0014-4827(90)90097-t.

Abstract

In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / isolation & purification
  • Kinetics
  • Methionine / metabolism
  • Molecular Weight
  • Peptide Mapping
  • RNA, Fungal / metabolism
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Fungal Proteins
  • RNA, Fungal
  • Methionine