Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains

J Proteomics. 2011 Nov 18;74(12):2846-55. doi: 10.1016/j.jprot.2011.06.032. Epub 2011 Jul 26.


A comparative proteomic approach, using two dimensional gel electrophoresis and mass spectrometry, has been developed to compare and elucidate the differences among the cellular proteomes of four closely related isogenic O/C, SIN, N/R and T, B. clausii strains during both exponential and stationary phases of growth. Image analysis of the electropherograms reveals a high degree of concordance among the four proteomes, some proteins result, however, differently expressed. The proteins spots exhibiting high different expression level were identified, by mass-spectrometry analysis, as alcohol dehydrogenase (ADHA, EC1.2.1.3; ABC0046 isoform) aldehyde dehydrogenase (DHAS, EC; ABC0047 isoform) and flagellin-protein of B. clausii KSM-k16. The different expression levels of the two dehydrogenases were confirmed by quantitative RT-PCR and dehydrogenases enzymatic activity. The different patterns of protein expression can be considered as cell proteome signatures of the different strains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / growth & development
  • Bacillus / metabolism*
  • Bacterial Proteins / biosynthesis*
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Gene Expression Regulation, Bacterial / physiology*
  • Mass Spectrometry / methods
  • Proteome / biosynthesis*
  • Proteomics / methods*


  • Bacterial Proteins
  • Proteome