The whole-blood hemoglobinometer is an anemia-screening tool commonly used in public health nursing practice. This study evaluated the reliability and validity of the instrument. Test-retest, intertester, and intertest reliability were all shown to be strong. Sensitivity was 1.0, specificity ranged from 0.8571 to 0.9024, positive predictive value ranged from 0.6428 to 0.7453, and negative predictive value was 1.0. A small but statistically significant difference in hemoglobinometer versus Coulter readings was found, resulting in a 10% false positive rate.