Topical grape (Vitis vinifera) seed extract promotes repair of full thickness wound in rabbit

Int Wound J. 2011 Oct;8(5):514-20. doi: 10.1111/j.1742-481X.2011.00833.x. Epub 2011 Aug 4.


In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Grape (Vitis vinifera) seed extract (GSE) possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential activity of grape seed hydroalcoholic extract in wound healing in rabbits. Rabbits of either sex were subjected to a 20 × 20 mm square excision made over the skin of the back. The animals were randomly divided into seven experimental groups, as negative and positive control, eucerin and treatments. Negative control group did not receive any treatment. Positive control and eucerin groups received phenytoin cream (1%) and topical eucerin, respectively, twice a day from the beginning of experiments to complete wound closure. Treatment groups were treated topically by cream of GSE (2, 5, 10 and 70% w/w) in eucerin base, twice daily. For evaluation of the percentage of wound healing, area of the wound was measured daily. Histological studies were performed on the 7th and 15th days of treatments. After complete healing, hydroxyproline content and tensile strength measurement of tissue samples were done. Results showed that there were statistically significant differences between GSE treatments groups and eucerin animals (P < 0·05) in most of the days. Rabbits treated with 2% GSE had best results (completed healing in 13 days, higher hydroxyproline content and higher tissue resistance). We concluded that the extract of 2% GSE administered topically has a good potential to promote wound healing in wound model of rabbits.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Disease Models, Animal
  • Female
  • Follow-Up Studies
  • Granulation Tissue / drug effects*
  • Granulation Tissue / pathology
  • Grape Seed Extract / administration & dosage*
  • Male
  • Rabbits
  • Treatment Outcome
  • Vitis
  • Wound Healing / drug effects*
  • Wounds and Injuries / drug therapy*
  • Wounds and Injuries / pathology


  • Grape Seed Extract