Real-time detection of β1 integrin expression on MG-63 cells using electrochemical impedance spectroscopy

Abstract

Beta 1 integrin is a membrane protein responsible for attachment and migration of osteosarcoma cells. In this study, expression of β1 integrin on MG-63 cells, a human osteogenic sarcoma cell line, was monitored using electrochemical impedance spectroscopy (EIS). ITO-based biochips were developed using a semiconductor technique. Differences in electric resistance (ΔR) were measured continuously when cells binding with anti-β1 integrin antibody coagulated with nano-scale gold particles. The results of the EIS system were compared with traditional immunofluorescence staining. We found that sample chambers with higher cell densities had larger ΔR values. When the cell densities increased from 5 × 10(4) cells/ml to 5 × 10(5) cells/ml, the ΔR value dose-dependently increased from 14 Ω to 37 Ω. In addition, a highly linear relationship (correlation coefficient, 0.921) was found between the ΔR values and the corresponding fluorescence intensities (p<0.05). These results suggest that electrochemical impedance spectroscopy can be a useful tool for evaluating β1 integrin expression on cell membranes.