Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit

Dev Cell. 2011 Aug 16;21(2):328-42. doi: 10.1016/j.devcel.2011.06.020. Epub 2011 Aug 4.


Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain localizes Repo-Man to bulk chromatin in early anaphase. There, it targets PP1 for the dephosphorylation of histone H3 and possibly other chromosomal substrates. An N-terminal domain localizes Repo-Man to the chromosome periphery later in anaphase. There, it is responsible for the recruitment of nuclear components such as Importin β and Nup153 in a PP1-independent manner. These observations identify Repo-Man as a key factor that coordinates chromatin remodeling and early events of nuclear envelope reformation during mitotic exit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase / genetics
  • Anaphase / physiology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Line, Transformed
  • Chromosomes / metabolism*
  • Cyclin B / metabolism
  • Gene Expression Regulation / physiology
  • Green Fluorescent Proteins / genetics
  • Histones / metabolism
  • Humans
  • Mitosis / genetics
  • Mitosis / physiology*
  • Models, Biological
  • Nuclear Envelope / physiology*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phosphorylation / physiology
  • Protein Binding / genetics
  • Protein Structure, Tertiary / physiology
  • RNA Interference / physiology
  • Receptors, Neuropeptide Y / genetics
  • Receptors, Neuropeptide Y / metabolism
  • Tandem Mass Spectrometry / methods
  • Transfection
  • beta Karyopherins / metabolism


  • CDCA2 protein, human
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclin B
  • Histones
  • Nuclear Proteins
  • Receptors, Neuropeptide Y
  • beta Karyopherins
  • Green Fluorescent Proteins
  • neuropeptide Y4 receptor