Negative autoregulation of c-myc transcription

EMBO J. 1990 Apr;9(4):1113-21.

Abstract

The introduction of activated c-myc and v-myc genes into a variety of non-established and established cells results in the suppression of endogenous c-myc expression. As measured in Rat-1 fibroblasts, the suppression occurs at the level of transcriptional initiation. Moreover, the extent of the down-regulation is proportional to the cellular concentration of c-myc protein, and the critical concentration range in which the endogenous c-myc RNA is effectively suppressed corresponds to that found in non-transformed cells. In addition, the autoregulatory mechanism is not only dependent on c-myc protein, but also requires additional trans-acting factors. These results support a role for c-myc in the regulation of cellular gene transcription and suggest that a negative feedback mechanism can act as a homeostatic regulator of c-myc expression in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Feedback
  • Gene Expression Regulation*
  • Homeostasis
  • Immunoblotting
  • Protein-Tyrosine Kinases / genetics
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogenes*
  • Rats
  • Restriction Mapping
  • Retroviridae / genetics
  • Ribonucleases
  • Suppression, Genetic*
  • Transcription, Genetic*
  • Transfection

Substances

  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myc
  • Protein-Tyrosine Kinases
  • Ribonucleases