γ-AApeptides bind to RNA by mimicking RNA-binding proteins

Org Biomol Chem. 2011 Oct 7;9(19):6604-9. doi: 10.1039/c1ob05738c. Epub 2011 Aug 8.

Abstract

The interactions between proteins and RNAs are of vital importance for many cellular processes, including transcription and processing of RNA, translation, and viral infections. Here we report an γ-AApeptide that can mimic HIV-1 Tat protein and bind to TAR RNAs of HIV and BIV with nanomolar affinity, comparable to that of the RNA-binding fragment of Tat (amino acids 49-58). The interaction is resistant to the presence of a large excess of tRNA. With resistance to proteolytic hydrolysis and limitless potential for diversification, γ-AApeptides may emerge as a new class of peptidomimetics to modulate RNA-protein interactions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Gene Products, tat / chemistry*
  • Molecular Mimicry
  • Molecular Structure
  • Peptides / chemical synthesis
  • Peptides / chemistry*
  • RNA, Viral / chemistry*
  • Stereoisomerism

Substances

  • Gene Products, tat
  • Peptides
  • RNA, Viral