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Randomized Controlled Trial
. 2011;6(7):e22748.
doi: 10.1371/journal.pone.0022748. Epub 2011 Jul 28.

Time-course of Changes in Inflammatory Response After Whole-Body Cryotherapy Multi Exposures Following Severe Exercise

Free PMC article
Randomized Controlled Trial

Time-course of Changes in Inflammatory Response After Whole-Body Cryotherapy Multi Exposures Following Severe Exercise

Hervé Pournot et al. PLoS One. .
Free PMC article

Erratum in

  • PLoS One. 2011;6(11). doi:10.1371/annotation/0adb3312-7d2b-459c-97f7-a09cfecf5881. Mounier, Rémi [added]


The objectives of the present investigation was to analyze the effect of two different recovery modalities on classical markers of exercise-induced muscle damage (EIMD) and inflammation obtained after a simulated trail running race. Endurance trained males (n = 11) completed two experimental trials separated by 1 month in a randomized crossover design; one trial involved passive recovery (PAS), the other a specific whole body cryotherapy (WBC) for 96 h post-exercise (repeated each day). For each trial, subjects performed a 48 min running treadmill exercise followed by PAS or WBC. The Interleukin (IL) -1 (IL-1), IL-6, IL-10, tumor necrosis factor alpha (TNF-α), protein C-reactive (CRP) and white blood cells count were measured at rest, immediately post-exercise, and at 24, 48, 72, 96 h in post-exercise recovery. A significant time effect was observed to characterize an inflammatory state (Pre vs. Post) following the exercise bout in all conditions (p<0.05). Indeed, IL-1β (Post 1 h) and CRP (Post 24 h) levels decreased and IL-1ra (Post 1 h) increased following WBC when compared to PAS. In WBC condition (p<0.05), TNF-α, IL-10 and IL-6 remain unchanged compared to PAS condition. Overall, the results indicated that the WBC was effective in reducing the inflammatory process. These results may be explained by vasoconstriction at muscular level, and both the decrease in cytokines activity pro-inflammatory, and increase in cytokines anti-inflammatory.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.


Figure 1
Figure 1. Study design - Recovery: PAS or WBC.
Figure 2
Figure 2. Changes in CRP (A), IL-1β (B) and IL-1ra (C) from post-running exercise to recovery.
#, significant difference between groups (p<0.05). WBC, whole body cryotherapy; PAS, passive rest recovery.

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