Cyclooxygenase-2 in Dukes B colon cancer

Hepatogastroenterology. May-Jun 2011;58(107-108):763-8.

Abstract

Background/aims: To demonstrate immunohistochemical expression of COX-2 protein in Dukes B colon cancer and to establish a correlation with clinicopathological parameters such as: age, gender, gradus, presence of vascular invasion and patient's overall survival.

Methodology: We performed immunohistochemical analysis of formalin-fixed, paraffin embedded specimens form 152 Dukes B colon carcinomas, using the COX-2 monoclonal antibody. Immunohistochemical results were scored semi-quantitatively. Carcinomas were graded as low or high grade. Survival time was analyzed by Kaplan-Meier method, and the log-rank test was used to assess the differences between groups. For multivariate analysis, Cox proportional hazard regression model was used to examine several parameters simultaneously.

Results: Univariate analysis showed that positive staining for COX-2, high histological grade; vascular invasion; male gender and age over 60 years, were connected with shorter survival of patients with Dukes B colon cancer (0.023< p<0.001). However, multivariate analysis have shown that the high COX-2 expression in Duke's B colon cancer was unrelated to overall patient survival (RR=1.4; p=0.311).

Conclusion: Expression of COX-2 in tumor epithelial cells does not seem to be related to survival in patients with colon cancer Dukes B.

MeSH terms

  • Adult
  • Aged
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / mortality
  • Cyclooxygenase 2 / analysis*
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Proportional Hazards Models

Substances

  • Cyclooxygenase 2
  • PTGS2 protein, human