Protection of SH-SY5Y Neuronal Cells From Glutamate-Induced Apoptosis by 3,6'-disinapoyl Sucrose, a Bioactive Compound Isolated From Radix Polygala

J Biomed Biotechnol. 2012;2012:1-5. doi: 10.1155/2012/728342. Epub 2011 Jun 30.

Abstract

The neuroprotective effects of 3,6'-disinapoyl sucrose (DISS) from Radix Polygala against glutamate-induced SH-SY5Y neuronal cells injury were evaluated in the present study. SH-SY5Y neuronal cells were pretreated with glutamate (8 mM) for 30 min followed by cotreatment with DISS for 12 h. Cell viability was determined by (3,4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay, and apoptosis was confirmed by cell morphology and flow cytometry assay, evaluated with propidium iodide dye. Treatment with DISS (0.6, 6, and 60 μmol/L) increased cell viability dose dependently, inhibited LDH release, and attenuated apoptosis. The mechanisms by which DISS protected neuron cells from glutamate-induced excitotoxicity included the downregulation of proapoptotic gene Bax and the upregulation of antiapoptotic gene Bcl-2. The present findings indicated that DISS exerts neuroprotective effects against glutamate toxicity, which might be of importance and contribute to its clinical efficacy for the treatment of neurodegenerative diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Coumaric Acids / isolation & purification
  • Coumaric Acids / pharmacology*
  • Drugs, Chinese Herbal / isolation & purification
  • Drugs, Chinese Herbal / pharmacology*
  • Flow Cytometry
  • Glutamic Acid / pharmacology
  • Humans
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Polygala / chemistry*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Sucrose / analogs & derivatives*
  • Sucrose / isolation & purification
  • Sucrose / pharmacology
  • bcl-2-Associated X Protein / metabolism

Substances

  • 3,6'-disinapoylsucrose
  • Coumaric Acids
  • Drugs, Chinese Herbal
  • Neuroprotective Agents
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • Glutamic Acid
  • Sucrose