The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae

EMBO J. 1990 May;9(5):1423-30.


The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl-terminal 21 amino acids present in the RAD6 protein are absent in the rhp6(+)-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA Repair / genetics
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics*
  • Genetic Complementation Test
  • Ligases / genetics*
  • Molecular Sequence Data
  • Mutation
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Nucleic Acid
  • Spores, Fungal / genetics
  • Ubiquitin-Conjugating Enzymes
  • Ultraviolet Rays


  • DNA, Fungal
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • RAD6 protein, S cerevisiae
  • Ubiquitin-Conjugating Enzymes
  • rhp6 protein, S pombe
  • Ligases