ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3

EMBO J. 1990 May;9(5):1437-47.

Abstract

Human ERCC2 genomic clones give efficient, stable correction of the nucleotide excision repair defect in UV5 Chinese hamster ovary cells. One clone having a breakpoint just 5' of classical promoter elements corrects only transiently, implicating further flanking sequences in stable gene expression. The nucleotide sequences of a cDNA clone and genomic flanking regions were determined. The ERCC2 translated amino acid sequence has 52% identity (73% homology) with the yeast nucleotide excision repair protein RAD3. RAD3 is essential for cell viability and encodes a protein that is a single-stranded DNA dependent ATPase and an ATP dependent helicase. The similarity of ERCC2 and RAD3 suggests a role for ERCC2 in both cell viability and DNA repair and provides the first insight into the biochemical function of a mammalian nucleotide excision repair gene.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Survival / genetics
  • Cloning, Molecular
  • DNA Repair / genetics*
  • Gene Library
  • Genes, Fungal / genetics*
  • Genetic Complementation Test
  • Humans
  • Introns / genetics
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic / genetics
  • Transformation, Genetic
  • Ultraviolet Rays

Associated data

  • GENBANK/X52221
  • GENBANK/X52222
  • GENBANK/X52470