Bβ(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation

J Am Soc Nephrol. 2011 Oct;22(10):1887-96. doi: 10.1681/ASN.2011010031. Epub 2011 Aug 12.


Renal ischemia-reperfusion contributes to reduced renal allograft survival. The peptide Bβ(15-42), a breakdown product of fibrin, attenuates inflammation induced by ischemia-reperfusion in the heart by competitively blocking the binding of leukocytes to endothelial VE-cadherin, but whether it could improve outcomes in renal transplantation is unknown. Here, we tested the ability of Bβ(15-42) to ameliorate the effects of renal ischemic injury during allogenic kidney transplantation in mice. In our renal transplantation model (C57BL/6 into BALB/c mice), treatment with Bβ(15-42) at the time of allograft reperfusion resulted in significantly improved survival of recipients during the 28-day follow-up (60% versus 10%). Bβ(15-42) treatment decreased leukocyte infiltration, expression of endothelial adhesion molecules, and proinflammatory cytokines. Treatment significantly attenuated allogenic T cell activation and reduced cellular rejection. Moreover, Bβ(15-42) significantly reduced tubular epithelial damage and apoptosis, which we reproduced in vitro. These data suggest that Bβ(15-42) may have therapeutic potential in transplant surgery by protecting grafts from ischemia-reperfusion injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury / prevention & control*
  • Animals
  • Apoptosis / drug effects
  • Epithelial Cells / drug effects
  • Female
  • Fibrin Fibrinogen Degradation Products / administration & dosage*
  • Kidney Transplantation / adverse effects*
  • Kidney Transplantation / immunology
  • Kidney Tubules / cytology
  • Major Histocompatibility Complex / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Peptide Fragments / administration & dosage*
  • Reperfusion Injury / prevention & control*
  • T-Lymphocytes / drug effects
  • Transplantation, Homologous


  • Fibrin Fibrinogen Degradation Products
  • Peptide Fragments
  • fibrinogen Bbeta (15-42)