Inhibition of renin release by arachidonic acid metabolites, 12(s)-HPETE and 12-HETE: role of TRPV1 channels

Endocrinology. 2011 Oct;152(10):3811-9. doi: 10.1210/en.2011-0141. Epub 2011 Aug 16.


We test the hypothesis that 12-hydroperoxyeicosatetraenoic acid (12(s)-HPETE) and 12-hydroxyeicosatetraenoic acid (12-HETE) perfused into the renal pelvis increase afferent renal nerve activity (ARNA) and suppress renin release in rats fed a low-salt (LS) diet via activation of the transient receptor potential vanilloid type 1 (TRPV1) expressed in renal sensory nerves. 12(s)-HPETE or 12-HETE given into the left renal pelvis dose-dependently increased ARNA, which was abolished by AMG9810, a selective TRPV1 antagonist, or by RP67580, a selective neurokinin 1 receptor antagonist, in normal salt or LS-treated rats. 12(s)-HPETE, 12-HETE, or substance P perfused into the left renal pelvis suppressed plasma angiotensin I (Ang I) levels in LS rats, which was abolished by AMG9810 or attenuated by ipsilateral renal denervation (RD). 12(s)-HPETE or 12-HETE increased release of substance P and calcitonin gene-related peptide from the ipsilateral kidney, which was abolished by AMG9810 but not RP67580, RD, or RP67580 plus RD. Immunofluorescence staining showed that TRPV1-positive nerve fibers located in the renal cortex, medulla, and pelvis, and that the sympathetic nerve marker, neuropeptide Y, but not neurokinin 1 receptors expressed in the juxtaglomerular region colocalized with renin. Thus, our data show that 12(s)-HPETE and 12-HETE enhance ARNA and substance P/calcitonin gene-related peptide release but suppress renin activity in LS rats, and these effects are abolished when TRPV1 is blocked. These results indicate that TRPV1 mediates 12(s)-HPETE and 12-HETE action in the kidney in such a way that dysfunction in TRPV1 may lead to disintegrated regulation of renin and renal function.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / pharmacology*
  • Angiotensin I / blood
  • Animals
  • Arachidonic Acid / metabolism
  • Leukotrienes / pharmacology*
  • Male
  • Rats
  • Rats, Wistar
  • Receptors, Neurokinin-1 / analysis
  • Renin / analysis
  • Renin / antagonists & inhibitors*
  • Renin / metabolism
  • TRPV Cation Channels / analysis
  • TRPV Cation Channels / physiology*


  • Leukotrienes
  • Receptors, Neurokinin-1
  • TRPV Cation Channels
  • Trpv1 protein, rat
  • Arachidonic Acid
  • 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
  • 12-HPETE
  • Angiotensin I
  • Renin