Imaging multiple conductance states in an alamethicin pore

J Am Chem Soc. 2011 Sep 21;133(37):14507-9. doi: 10.1021/ja204275t. Epub 2011 Aug 24.

Abstract

Alamethicin is the archetypal antimicrobial pore-forming peptide. Although the peptide has long been known to form pores of characteristic conductances in lipid membranes, the precise nature of these pores is not known. Simultaneous calcium-flux imaging and single-channel recording in a droplet interface bilayer allowed us to directly attribute multiple conductance states to a single point diffusing in the bilayer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alamethicin / chemistry
  • Alamethicin / metabolism*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / metabolism*
  • Calcium / metabolism*
  • Electric Conductivity
  • Electrochemical Techniques
  • Lipid Bilayers / chemistry
  • Lipid Bilayers / metabolism

Substances

  • Anti-Bacterial Agents
  • Lipid Bilayers
  • Alamethicin
  • Calcium