Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves

FEBS Lett. 2011 Oct 3;585(19):3006-10. doi: 10.1016/j.febslet.2011.08.016. Epub 2011 Aug 16.


Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxygen species (ROS) levels and cell death in response to specific sphingobases. t18:0, d18:0 and d17:1, but not d20:0, induced ROS and cell death within 1.5-2h. Early sphingobase-induced ROS production was independent of cell death induction and required the NADPH oxidase Respiratory Burst Oxidase Homolog D (RBOHD). Specific sphingobases can therefore induce cell death and require RBOHD for early ROS induction in plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / anatomy & histology*
  • Arabidopsis / metabolism*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Death / physiology
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • NADPH Oxidases / genetics
  • NADPH Oxidases / metabolism*
  • Plant Leaves / metabolism*
  • Pseudomonas syringae / metabolism
  • Pseudomonas syringae / pathogenicity
  • Reactive Oxygen Species / metabolism*
  • Signal Transduction / physiology
  • Sphingolipids / chemistry
  • Sphingolipids / metabolism*


  • Arabidopsis Proteins
  • Isoenzymes
  • Reactive Oxygen Species
  • Sphingolipids
  • respiratory burst oxidase homolog D, Arabidopsis
  • NADPH Oxidases
  • superoxide-forming enzyme