Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice

Vaccine. 2011 Oct 19;29(45):8134-40. doi: 10.1016/j.vaccine.2011.08.030. Epub 2011 Aug 19.


Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis larvae in mice. Alum was used as the adjuvant in these studies and antigens selected for analysis were either recognized by protective human IgG (Ss-TMY-1, Ss-EAT-6, and Ss-LEC-5) or were known to be highly immunogenic in humans (Ss-NIE-1 and Ss-IR). Only mice immunized with the Ss-IR antigen demonstrated a significant decrease of approximately 80% in the survival of larval parasites in the challenge infection. Antibodies, recovered from mice with protective immunity to S. stercoralis after immunization with Ss-IR, were used to locate the antigen in the larvae. Confocal microscopy revealed that IgG from mice immunized with Ss-IR bound to the surface of the parasites and observations by electron microscopy indicated that IgG bound to granules in the glandular esophagus. Serum collected from mice immunized with Ss-IR passively transferred immunity to naïve mice. These studies demonstrate that Ss-IR, in combination with alum, induces high levels of protective immunity through an antibody dependent mechanism and may therefore be suitable for further development as a vaccine against human strongyloidiasis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Alum Compounds / administration & dosage
  • Animal Structures / immunology
  • Animals
  • Antibodies, Helminth / immunology
  • Antigens, Helminth / administration & dosage
  • Antigens, Helminth / immunology*
  • Larva / immunology
  • Larva / ultrastructure
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Strongyloides stercoralis / immunology*
  • Strongyloides stercoralis / ultrastructure
  • Strongyloidiasis / mortality
  • Strongyloidiasis / prevention & control*
  • Survival Analysis


  • Adjuvants, Immunologic
  • Alum Compounds
  • Antibodies, Helminth
  • Antigens, Helminth
  • aluminum sulfate