[The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Apr;25(2):152-4.
[Article in Chinese]

Abstract

Objective: To establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid.

Methods: Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.

Results: The real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods.

Conclusion: The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • DNA Primers / chemistry
  • DNA Primers / genetics
  • Enterovirus A, Human / genetics
  • Enterovirus A, Human / isolation & purification*
  • Female
  • Fluorescent Dyes / chemistry
  • Hand, Foot and Mouth Disease / diagnosis*
  • Hand, Foot and Mouth Disease / pathology
  • Hand, Foot and Mouth Disease / virology
  • Humans
  • Infant
  • Male
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Primers
  • Fluorescent Dyes