Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection

Clin Vaccine Immunol. 2011 Oct;18(10):1773-5. doi: 10.1128/CVI.05288-11. Epub 2011 Aug 24.

Abstract

A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphavirus Infections / diagnosis*
  • Antibodies, Viral / blood*
  • Chikungunya Fever
  • Chikungunya virus / immunology*
  • Clinical Laboratory Techniques / methods*
  • Cohort Studies
  • Humans
  • Immunoassay / methods
  • Immunoglobulin M / blood*
  • Sensitivity and Specificity
  • Sri Lanka

Substances

  • Antibodies, Viral
  • Immunoglobulin M