Due to its ability of hydrolyzing alpha-1,6-linkages, pullulanase plays a significant role in the industries where starch is used as raw material, including food processing and bioenergy. Exploitation of this enzyme focuses not only on screening novel strains, but also on its molecular structure as well as its secretion pathway and improvement of the properties. Therefore, researches on the gene regulation and the proteins involved in the secretory pathway seem especially important. This review introduced the advances in the study of these fields.