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The Real Cost of Sequencing: Higher Than You Think!


The Real Cost of Sequencing: Higher Than You Think!

Andrea Sboner et al. Genome Biol.


Advances in sequencing technology have led to a sharp decrease in the cost of 'data generation'. But is this sufficient to ensure cost-effective and efficient 'knowledge generation'?


Figure 1
Figure 1
Contribution of different factors to the overall cost of a sequencing project across time. Left, the four-step process: (i) experimental design and sample collection, (ii) sequencing, (iii) data reduction and management, and (iv) downstream analysis. Right, the changes over time of relative impact of these four components of a sequencing experiment. BAM, Binary Sequence Alignment/Map; BED, Browser Extensible Data; CRAM, compression algorithm; MRF, Mapped Read Format; NGS, next-generation sequencing; TAR, transcriptionally active region; VCF, Variant Call Format.
Figure 2
Figure 2
Cost of 1 MB of DNA sequencing. Decreasing cost of sequencing in the past 10 years compared with the expectation if it had followed Moore's law. Adapted from [11]. Cost was calculated in January of each year. MB, megabyte.

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