Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range

Mar Genomics. 2011 Sep;4(3):181-8. doi: 10.1016/j.margen.2011.04.003. Epub 2011 Jun 14.


Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30min, 2h, 4h and 24h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Evolution
  • Climate Change
  • Demography
  • Expressed Sequence Tags*
  • Flavoproteins / genetics
  • Flavoproteins / metabolism
  • Gene Expression Regulation, Plant / physiology*
  • Glutamate-Ammonia Ligase / genetics
  • Glutamate-Ammonia Ligase / metabolism
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Hot Temperature*
  • Microsatellite Repeats
  • Oxidative Stress
  • Oxidoreductases Acting on CH-CH Group Donors / genetics
  • Oxidoreductases Acting on CH-CH Group Donors / metabolism
  • Peptidylprolyl Isomerase / genetics
  • Peptidylprolyl Isomerase / metabolism
  • Photosynthesis / physiology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Transcriptome
  • Zosteraceae / genetics
  • Zosteraceae / metabolism*


  • Flavoproteins
  • Plant Proteins
  • vestitone reductase
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Oxidoreductases Acting on CH-CH Group Donors
  • Peptidylprolyl Isomerase
  • Glutamate-Ammonia Ligase