A sensitive and reproducible method for the assay of human islet cell antibodies

J Immunol Methods. 1990 May 8;129(1):111-7. doi: 10.1016/0022-1759(90)90427-w.


The islet cell antibody test is increasingly used to predict insulin-dependent diabetes before it is clinically apparent. Four international workshops on the test have shown wide variations in reproducibility, sensitivity and specificity. This illustrates the need for a more clearly defined method. We report a technique which offers both increased sensitivity and reproducibility. Using this method 94% of newly diagnosed diabetic children have ICA levels greater than or equal to 10 international units, whereas only 1.5% of normal children are positive at his level, and 7% of first degree relatives (aged less than 20 years) of IDDM patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Autoantibodies / analysis*
  • Child
  • Child, Preschool
  • Diabetes Mellitus, Type 1 / diagnosis*
  • Fluorescent Antibody Technique
  • Humans
  • Infant
  • Islets of Langerhans / immunology*
  • Middle Aged
  • Predictive Value of Tests
  • Reproducibility of Results


  • Autoantibodies
  • islet cell antibody