Stable-isotope Labeling With Amino Acids in Nematodes

Nat Methods. 2011 Aug 28;8(10):849-51. doi: 10.1038/nmeth.1679.

Abstract

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / chemistry*
  • Arginine / metabolism
  • Caenorhabditis elegans / metabolism*
  • Cells, Cultured
  • Escherichia coli / chemistry
  • Heat-Shock Response / physiology
  • Isotope Labeling / methods*
  • Lysine / chemistry*
  • Proline / chemistry
  • Proline / metabolism
  • Proteomics

Substances

  • Arginine
  • Proline
  • Lysine